ENRICH
Biosystems

Applications

One platform.
A spectrum of high-throughput workflows.

TROVO supports functional screening, longitudinal imaging, clone selection, live cell enrichment and recovery — across immunotherapy, antibody discovery, single-cell omics and cell line development.

Application 01

TIL Discovery Workflow

Complete workflow for TIL-based immunotherapy — from tumor dissociation through microwell seeding, longitudinal co-culture imaging, functional clone selection and live cell recovery. Designed for clinical manufacturing where sample scarcity, reproducibility and process control are critical.

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Key benefits

  • End-to-end workflow from tissue to functional clone
  • Function-first selection — no marker assumptions
  • Preserves transcriptome integrity
  • Fluidics-free capture with no shear stress
Application 02

TIL Potency Assessment

Identify and quantify tumor-killing TILs from clinical samples using Z-score-based potency assessment. Each co-culture microwell is compared against matched tumor-only controls, generating statistically rigorous killer-clone metrics from small patient samples.

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Key benefits

  • Potency assessment from ~10,000 cells
  • Marker-free responder / non-responder discrimination
  • Matched internal controls on the same plate
  • Compatible with recovery, sequencing, expansion & re-challenge
Application 03

CAR-T & TCR-T Clone Selection and Kinetics

Rank CAR-T and TCR-T constructs by real-time killing kinetics — not surface markers. TROVO tracks cytotoxicity and persistence over extended assays, separating true persistent killers from short-term burst killers before downstream investment.

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Key benefits

  • Live tracking of cytotoxicity and persistence
  • Longitudinal clone comparison over multiple days
  • Functional Z-score ranking
  • Recovery of viable clones for NGS, expansion and validation
Application 04

Predator–Prey Kinetics

Per-clone kinetic data can be fit to Lotka–Volterra predator–prey equations to derive quantitative killing rate constants for each clone — separating intrinsic single-cell killing from cooperative killing at higher effector densities.

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Key benefits

  • Quantitative killing rate constants
  • Separation of intrinsic vs cooperative tumor killing
  • High-resolution clone-level kinetic profiling
  • Correlation with in vivo tumor control
Application 05

High-Throughput B Cell Screening for Antibody Discovery

Image individual B cell IgG secretion over time and recover cells of interest. Thousands of hydrogel microwells on a standard 6-well plate provide controlled microenvironments; B cells can be incubated with antigen-coated beads or target cells and labeled with secondary antibodies to detect secretion within hours.

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Key benefits

  • Detection of rare secretors
  • Secretion monitoring over time
  • Recovery for sequencing, expansion or validation
  • Compatible with peptide, bead-based and native cell targets
Application 06

T Cell Clone Selection Based on Tumor Co-Cultures

Optimize T cell clone selection using hydrogel microwells and imaging to track T cell–tumor interactions. Identify top-performing clones for CAR-T and TCR-T development with a workflow that completes in approximately 12 days.

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Key benefits

  • Functional clone identification
  • Live cell isolation for downstream analysis
  • Expansion and validation of recovered cells
  • ~12-day end-to-end workflow
Application 07

Time-Lapse Microculture AI Analysis

TROVO can automatically group microculture movies into function-based categories with minimal human interaction. AI-assisted grouping distinguishes promising T cell clones from undesirable ones, reducing user variability and eliminating the need for complex manual segmentation strategies.

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Key benefits

  • Automated microculture movie classification
  • Function-based grouping
  • Reduced user bias
  • Less dependence on manual segmentation
Application 08

Live Cell Enrichment for Single-Cell RNA-seq

Support live cell enrichment for single-cell RNA sequencing using light-induced microgel lithography and image-based selection. Gently isolates live cells from fresh or frozen tissue samples while preserving viability and transcriptomic integrity.

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Key benefits

  • Fluidics-free — no clogging, low shear stress
  • Higher transcriptome integrity
  • Up to three samples in four hours
  • Compatible with 10x Genomics workflows

Get in touch

Which workflow are you optimizing?

Tell us about your application — cell type, target, throughput, downstream. Our scientific team will map TROVO to your experimental context.

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