Data Hub
Publications, case studies and webinars showing how researchers use TROVO to move beyond marker dependency and noisy bulk readouts.
Featured publication
Katz Lab, Yale University — Preprint, January 2026. Chen PH, Li Q, Deveraux S, Sohai D, Cha PC, Raghunandan R, Chen N, Li Y, Nguyen M, Stankewich MC, Morrow JS, Augert A, Yan QC, Katz SG.
The study evaluated MET-targeting VHH CAR-T cells as an alternative to conventional scFv-based CAR-T constructs. TROVO was used to evaluate hydrogel microwell-based cellular kinetics and track real-time cytotoxicity across thousands of individual co-cultures.
Intermediate-avidity clones outperformed high-avidity clones in vitro — a finding enabled by per-clone kinetic tracking.
VHH-CAR-T cells showed superior biochemical stability and favorable cytokine profiles vs traditional scFv-based designs.
TROVO microwell co-cultures enabled direct measurement of cytotoxicity and T cell behavior across thousands of interactions.
VHH-CAR-T cells demonstrated potent and prolonged tumor growth control in metastatic triple-negative breast cancer models.
Case studies
Distinguishing responders from non-responders
TROVO seeds patient TILs and tumor cells into thousands of microwells, tracks killing longitudinally and compares each co-culture against matched tumor-only controls — enabling statistically rigorous responder discrimination from scarce clinical samples.
From tissue to functional clone recovery
Dissociate → seed → image (2–5 days) → rank by Z-score → capture → wash → enzymatic release → NGS / expansion / re-challenge. A clinical-manufacturing-compatible, function-first workflow with intact transcriptome after recovery.
12-day persistence assays with tumor re-challenge
TROVO tracks cytotoxicity and persistence kinetics over 12 days with tumor re-challenge every three days, ranking clones by functional Z-score and capturing viable populations for downstream NGS and expansion.
Intrinsic vs cooperative tumor killing
Per-clone data fit to Lotka–Volterra equations yields clone-specific killing rate constants, separating intrinsic killing capacity from cooperative wolf-pack killing — with in vivo correlation in NSG mouse models.
Two-round multi-signal workflow
Identify B cells that bind both target peptide and full-length native protein — from PBMC seeding and streptavidin bead coating through photo-crosslinked capture, wash and enzymatic release for RT-PCR and Sanger sequencing.
Image-guided, fluidics-free tissue processing
From raw tissue to single-cell suspension in ~4 hours for three samples, using only ~10⁴ cells. No clogging, no shear stress, improved reads-in-cells and preserved tissue-specific features.
On-demand webinars
Not all CRISPR hits are equal. Some edits drive proliferation while others enhance cytotoxicity or persistence. Functional resolution at the single-well level reveals what bulk screens miss.
Request accessThe DEFINE workflow, myeloid heterogeneity, high-plex Imaging Mass Cytometry and TROVO-enabled functional co-culture assays — linking real-time killing kinetics to high-plex proteomic data.
Request accessCAR-T clone selection in 2D and 3D brain tumor co-culture models, including glioblastoma — with AI-driven kinetic analysis supporting scalable CAR-T development workflows.
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